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K-12 MG1655 Strain
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MP1457

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产品名称: K-12 MG1655 Strain
商品货号: MP1457
规格: 100μl
储存: -20℃
基因型: F- lambda- ilvG- rfb-50 rph-1(NC_000913)
基本信息: 抗性:无 培养基:LB 菌株类别:大肠杆菌 培养条件:37℃,有氧,LB 质粒转化:42℃热激 保存方式:20%甘油,-20℃ 基本应用:用于基因克隆 备注:
菌株简介: K-12 MG1655是一株K系列的大肠杆菌,经检测无Amp和Kan抗性,可用LB培养基,37℃培养,其代谢类型是异养兼性厌氧型,可用于代谢研究、基因编辑等实验。 The original K-12 wild-type strain from the Stanford collection contained both the F plasmid and phage lambda. MG1655 was constructed by curing that strain using acridine orange and UV.This strain was sequenced by the Blattner laboratory because it approximates wild-type E. coli and "has been maintained as a laboratory strain with minimal genetic manipulation, having only been cured of the temperate bacteriophage lambda and F plasmid by means of ultraviolet light and acridine orange, respectively." (Blattner, et al. 1997). The mutations listed in the genotype are present in most K-12 strains and were probably acquired early in the history of the laboratory strain. A frameshift at the end of rph results in decreased pyrE expression and a mild pyrimidine starvation, such that the strain grows 10 to 15% more slowly in pyrimidine-free medium than in medium containing uracil (Jensen 1993). The ilvG- mutation is a frameshift that knocks out acetohydroxy acid synthase II (Lawther, et al. 1982). The rfb-50 mutation is an IS5 insertion that results in the absence of O-antigen synthesis (Liu and Reeves 1994). MG1655 was derived and named by Mark Guyer from strain W1485, which was derived in Joshua Lederberg's lab from a stab-culture descendant of the original K-12 isolate. This original E. coli strain K-12 was obtained from a stool sample of a diphtheria patient in Palo Alto, CA in 1922 (Bachmann, B., pp. 2460-2488 in Neidhardt et al.1996, Escherichia coli and Salmonella: Cellular and Molecular Biology, ASM Press).MG1655 grows on LB and M9 minimal medium (+ Glucose + 1ug/ml thiamine).